Molecular Dynamics Analysis of Thermostable DNA Pol I ITB-1
نویسندگان
چکیده
منابع مشابه
The Role of Interface Domain Interactions on Thermal Stability of DNA polymerase I ITB-1
Temperature-induced unfolding of Klenow-like DNA Pol I ITB-1 was investigated by molecular dynamic simulation, focusing on the key factors that stabilizing the protein. The result showed that the protein unfolded initially by disruptions of the interface between of 5’ 3’polymerase and 3’ 5’ exonuclease domains. Several amino acid residues, Lys374-Glu489 and Lys381-Glu487, form salt bridges at t...
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Pol I is the most abundant polymerase in E. coli and plays an important role in short patch repair. In accord with this role in the cell, the purified polymerase exhibits low processivity and high fidelity in vitro. Pol I is also the polymerase responsible for leader strand synthesis during ColE1 plasmid replication. In a previous publication, we described the generation of a highly error-prone...
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ژورنال
عنوان ژورنال: MIcrobiology Indonesia
سال: 2007
ISSN: 1978-3477,2087-8575
DOI: 10.5454/mi.1.3.1